Read e-book online Enzymology primer for recombinant DNA technology PDF

By Hyone-Myong Eun

ISBN-10: 0122437403

ISBN-13: 9780122437403

Enzymes are fundamental instruments in recombinant DNA know-how and genetic engineering. This booklet not just presents info for enzymologists, yet does so in a fashion that might additionally reduction nonenymologists in making right use of those biocatalysts of their learn. The Enzymology Primer for Recombinant DNA expertise contains info no longer often present in the short descriptions given in so much books on recombinant DNA method and gene cloning. Key beneficial properties* presents crucial fundamentals in addition to updated details on enzymes most typically utilized in recombinant DNA expertise* offers info in an simply available structure to function a brief reference resource* results in a greater knowing of the function of biocatalysts in recombinant DNA concepts

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The compounds that structurally resemble true substrates but cannot be "turned over" by the enzyme are called pseudo-substrates, substrate analogs, or inhibitors. The relative magnitude of binding affinities for a series of substrates and/or analogs often reflects the physical dimension and physicochemical nature of the active site and enzyme-substrate interactions. , transition-state analogs) bind more tightly than the substrates, resulting in "specific" inhibition of the enzyme. The vanadyl ribonucleoside complex (VRC) is a good example of a "transition-state" inhibitor for RNase A.

Errors occur at the RNA level as well due to incorporation of wrong nucleotides during transcription catalyzed by RNA polymerase(s). In fact, errors arising from transfer of mutated genetic information are more frequent than errors introduced during translation. Spontaneous mutations occur at a frequency of 10"^-10"^ per locus per generation due to misincorporation by DNA (or RNA) polymerases. Random and sometimes programmed amino acid substitutions and unusual elongations occur during translation at frequencies ranging from a few percent to as low as 10"^ per event (44).

In addition to the two ordered secondary structures, a polypeptide chain may also contain nonregular structures often classified as random coil. The random coils, which include turns and loops, also play a significant role in the function of a protein. One notable substructure is a ^ bend or turn in which 3 - 5 successive residues are involved as a unit to sharply change the direction of the main chain. A loop is a continuous segment of a polypeptide chain that connects other defined structures.

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Enzymology primer for recombinant DNA technology by Hyone-Myong Eun

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