By Carl Wu, C. David Allis
DNA within the nucleus of plant and animal cells is saved within the kind of chromatin. Chromatin and the Chromatin remodelling enzymes play a big function in gene transcription.
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Additional resources for Chromatin and Chromatin Remodeling Enzymes, Part B
Tech. 49, 292 (2000). 7  visualization and 3D structure determination 37 in negative stain, this is in the range of 10–15 e/A2 per exposure. Many samples can tolerate up to 25 e/A2. Some proteins and DNA-protein complexes, dependent on a poorly understood combination of the preparative method and the negative stain, are extremely sensitive to damage. The migration of stain molecules, producing granulation, is usually obvious to the eye, but damage to the biological material is subtler. If the microscope is equipped with a CCD camera for digital data recording, it is useful to collect a series of extremely low-dose images of a single field, until the cumulative dose is $25 e/A2.
376 Copyright 2004, Elsevier Inc. All rights reserved. 00 48  chromatin proteins computationally with the typically large data sets involved. An ideal computational visualization environment would permit access to the greyscale data while having a transparent volume to interactively move on arbitrary as well as orthogonal axes, and incorporate 3D measurement tools. While freeware volume visualization packages are available, currently each falls short in some aspect. com). National Resources available in the United States.
Com). com). tvips. edu). . com). Electron Microscope Data Collection CCD Cameras Versus Film The theoretical pros and cons of digital versus film recording of data have been extensively discussed8; here we will consider some of the practical considerations. For beam-sensitive specimens (negatively stained or frozen hydrated) the microscope must be able to record low-dose images via deflection/shuttering of the beam above the sample. For most samples 6 J. Bednar and C. L. Woodcock, Meth. Enz. 304, 191 (1999).
Chromatin and Chromatin Remodeling Enzymes, Part B by Carl Wu, C. David Allis
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