By Kenneth Sauer (Eds.)
This quantity comprises spectroscopic equipment for the characterization of macromolecules. tools span the electromagnetic spectrum from X-ray to microwaves. It makes a speciality of the categories of knowledge that may be derived: how measurements are made; state-of-the-art gear; information acquisition, research, and interpretation; casebook examples; and new advancements and destiny instructions.
* Ultraviolet and visual Spectroscopy, including
* Absorption and round Dichroism
* temporary Absorption and Kinetics
* Linear Dichroism and Fluorescence
* Vibrational Spectroscopy
* Magnetic Resonance Spectroscopy
* X-Ray Spectroscopy
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Extra info for Biochemical Spectroscopy
A. Tobey, and C. Bustamante, Biochemistry 24, 5152 (1985). 18 L. D. Barron, M. P. Bogaard, and A. D. Buckingham, J. Am. Chem. Soc. 95, 603 (1973). 14] CIRCULARDICHROISM 39 surements on biopolymers feasible. 19 These and other developments in ROA have been reviewed, z°'z~ Theoretical A s p e c t s The theoretical basis of CD is discussed in several reviews. 22-25 A quantity called the rotational strength, which is a property of a given electronic or vibrational transition, generally provides the connection between theory and experiment in CD.
A. Nafie, Topics Stereochem. 17, 113 (1987). T. A. Keiderling, in "Practical Fourier Transform Infrared Spectroscopy" (J. R. Ferraro and K. ), p. 203. Academic Press, San Diego, 1990. T. A. Keiderling, in "Circular Dichroism: Interpretation and Applications" (K. Nakanishi, N. Berova, and R. W. ), p. 497. VCH, New York, 1994. ~0 K. Imahori and N. A. Nicola, in "Physical Principles and Techniques of Protein Chemistry" (S. J. ), Part C, p. 357. Academic Press, New York, 1973. i1 j. T. Yang and T. Samejima, Prog.
M. Gray, R. L. Ratliff, V. P. Antao, and C. W. Gray, in "Structure and Expression, Volume 2: DNA and Its Drug Complexes" (M. H. Sarma and R. H. ), p. 147. Adenine Press, Schenectady, New York, 1988. [3l 31 ABSORPTION AND CD OF NUCLEIC ACIDS existence of self-complexes of the individual strands may hinder or prevent the formation of stoichiometric, paired complexes. Characterization of Hybrids and Triplexes by Circular Dichroism Spectra CD spectra contain more features than do absorption spectra, which makes CD spectra especially useful in distinguishing and characterizing the conformations of different oligomer complexes.
Biochemical Spectroscopy by Kenneth Sauer (Eds.)
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