Download e-book for kindle: Atomic Force Microscopy in Cell Biology by Bhanu P. Jena

By Bhanu P. Jena

ISBN-10: 0123838517

ISBN-13: 9780123838513

ISBN-10: 0125441711

ISBN-13: 9780125441711

This is often the 1st e-book to hide the background, constitution, and alertness of atomic strength microscopy in mobilephone biology. awarded within the transparent, well-illustrated sort of the equipment in mobile Biology sequence, it introduces the AFM to its readers and allows them to faucet the facility and scope of this expertise to additional their very own examine. a realistic laboratory consultant to be used of the atomic strength and photonic strength microscopes, it presents up to date expertise and techniques in strength spectroscopy. it's also a accomplished and easy-to-follow functional laboratory consultant for using the AFM and PFM in organic learn.

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Extra resources for Atomic Force Microscopy in Cell Biology

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The preparation of large acinar cell clusters (10–15 cells), having intact tight junction, demonstrated the presence of microvilli with interspersed pits and depressions (Fig. 6). Analogous to a cornfield with potholes, the microvilli and pits could be observed at the apical end of live pancreatic acinar cells. When transmission electron microscopy was performed on these cells, the presence of microvilli was confirmed 40 Jena and Cho Fig. 3 Topology of the apical cell surface of isolated pancreatic acini, observed using AFM.

Appl. Phys. Lett. 40, 178. , Quate, C. , and Gerber, Ch. (1986). Phys. Rev. Lett. 56, 930. Corey, D. , and Hudspeth, A. J. (1983). J. Neurosci. 3, 962. Crevel, I. -T. , and Cross, R. A. (1996). J. Mol. Biol. 257, 66. , and H¨orber, J. K. H. (1998). Cell Biol. Intern. 21, 707. , H¨orber, J. K. , and Stelzer, E. H. K. (1996). Appl. Phys. Lett. 69, 446. , Moy, V. , and Gaub, H. E. (1994). Science 264, 415. , H¨orber, J. K. , and Stelzer, E. H. K. (1997). J. Struct. Biol. 119, 202. , Stelzer, E. H.

Depending on the application, one or both of these detection systems are used. At room temperature, the thermal position fluctuations of the trapped bead in weak trapping potentials reach several hundred nanometers. At first glance, the fluctuation seems to be disturbing noise that limits the resolution. However, due to the speed and resolution of the position sensor based on the forward-scattered light detection, the fluctuations of the bead can be tracked directly, presenting new methods of analyzing the interaction of the bead with its environment.

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Atomic Force Microscopy in Cell Biology by Bhanu P. Jena

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