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By Bhanu P. Chowdhary

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1998). A missense mutation (Lys232 → Ala) in DGAT1 has been shown to be significantly associated with variation in milk fat percentage in cattle. , 2002). , 2000) revealed that DGAT-like activity is found in other enzymes encoded by other genes and led to the detection of DGAT2. In humans, DGAT2 is expressed in many tissues. , 2001). , 2001). To date, this family has not been fully characterized in any single mammalian species. As such, the nomenclature for the family has not been finalized. This is especially the case with those members encoding monoacylglycerol acytransferase activity.

2002) and 28 loci were mapped to both the linkage and WGRH map. The WGRH and linkage mapping results together with the BAC mapping results and information from the human draft sequence were used to assign a putative order of loci along BTA19 and thus deduce the number and position of evolutionary breakpoints between bovine and human genomes. htm), was used for the majority of this study. This library was prepared from genomic DNA isolated from white blood cells of a Hereford bull, L1 Domino 99375 (USDA-ARS, Miles City, MT), and partially digested with MboI.

The consensus sequence of the two ESTs for MOGAT1 covers exons 1–3 and 6. Bovine ESTs were assembled into consensus mRNA sequences. Human and mouse sequence data (sources: NCBI, http://www. org/) were used to obtain putative splice sites. PCR primers were designed using Primer3 software (Rozen and Skaletsky, 1998) and considering splice sites and intron sizes in humans. PCR primers for MOGAT1 exons 4 and 5 were derived from the human cDNA sequence (GenBank accession no. AF384163). Initial PCR primers for MOGAT2 were derived from a porcine MOGAT2 EST sequence (GenBank accession no.

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Animal genomics, Volume 102 by Bhanu P. Chowdhary

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