By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)
Animal mobile expertise is a transforming into self-discipline of phone biology which goals not just to appreciate buildings, services and behaviors of differentiated animal cells, but additionally to check their talents for use for business and scientific reasons. The objective of animal mobile expertise contains the clonal growth of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of scientific and pharmaceutical importantance, and the appliance of animal cells to gene treatment, man made organs and the construction of practical meals. This quantity supplies the readers a whole assessment of the current cutting-edge and should be helpful for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, relatively cellphone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines with regards to animal phone culture.
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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006
Journal of Molecular Endocrinology 8 (3): 213–223. 5. , Negrete-Virgen, J. , Lyddiatt, A. and Al-Rubeai, M. (2002) Rapid Titration of Adenoviral Infectivity by Flow Cytometry in Batch Culture of Infected HEK 293 Cells. Cytotechnology 38: 87–97. Simple and Efficient Establishment of Recombinant Protein Hyper-Producing Cells by Using RAS-Amplified CHO Cell Line Tsukasa Fujiki, Toshiki Matsuo, Makiko Yamashita, Yoshinori Katakura, Shin-Ei Matsumoto, Kiichiro Teruya, and Sanetaka Shirahata Abstract Chinese hamster ovary (CHO) cells is a widely used host cell line and can massproduce recombinant proteins by using various amplifiable selectable marker gene and selection drugs.
Keywords Cryopreservation • hybridoma • insulinoma • sericin • serum-free 1 Introduction Mammalian cell culture is applicable to many fields. Various biological substances are produced as medicines by cultured mammalian cells, because of their high bioactivity due to glycosylation. In cell culture, the risk of contamination by bacteria, fungi, mycoplasmas and viruses is always a concern. In addition, cell function is impaired gradually during the culture period. During cryopreservation, cells arrest their metabolic pathways, therefore making it possible to maintain their functions.
Bioprocess International 56–64. 3. Sunstrom, N. , Sugyiono, D. , and Gray, P. (1998) Recombinant Insulin-Like Growth Factor-I (IGF-1) Production in Super-CHO Results in the Expression of IGF-1 receptor and IGF Binding Protein 3. Cytotechnolgy 28: 91–99. 4. Francis, G. , Ballard, F. , Milner, S. , McNeil, K. , Wallace, J. , King, R. and Wells, J. R. (1992) Novel Recombinant Fusion Protein Analogues of Insulin-Like Growth Factor (IGF)-I Indicate the Relative Importance of IGF-Binding Protein and Receptor Binding for Enhanced Biological Potency.
Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 by Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)