Read e-book online Animal Cell Culture and Technology (THE BASICS (Garland PDF

By Michael Butler

ISBN-10: 1859960499

ISBN-13: 9781859960493

This e-book is a wonderful source for college kids and starting clinical reaseraches. it's a stable resource of simple tools and strategies for winning laboratory cellphone tradition systems. lots of the present mobilephone tradition tools are coated yet this assurance isn't very particular. back, first-class for newbies.

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Extra resources for Animal Cell Culture and Technology (THE BASICS (Garland Science))

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This may originate from surface-active proteins secreted by the cells or from a serum supplement in the Basic equipment and laboratory design 39 growth medium (Grinnell, 1978). The polystyrene used to make most domestic plastic containers is unsuitable as a surface for cell attachment because it is hydrophobic and has no charge. The ‘tissue culture-grade’ plasticware available from commercial sources is generally made of polystyrene, which is treated to allow a negative surface charge (Maroudas, 1977).

Incubator can cause corrosion of the internal mechanical drives. g. Bellco), which are sealed units placed inside the humidified incubator. A separate master controller that can control several stirrer modules is placed outside the incubator. 11a). The three standard sizes of roller bottles offer a growth surface of 490, 850 or 1750 cm2 and can be filled with 250–500 ml of medium. 11b). On inoculation into the culture medium, the cells attach and grow over the entire inner surface. The bottles are positioned to turn slowly along the long axis at between 5–60 revolutions per hour (rph).

2002) Directed differentiation of embryonic stem cells into motor neurons. Cell 110:385– 397. M. J. (1997) Cytokine manipulation of primitive human hematopoietic cell self-renewal. Proc. Natl Acad. Sci. (USA) 94:4698–4703. I. (2000) Culture of Animal Cells: A manual of basic technique, 4th Edn. R. Liss, New York. M. T. (1996) Hematopoietic cell culture therapies (Part 1): cell culture considerations. Trends Biotechnol. 14: 341–349. The following are two accounts of the procedures for handling cells in culture collections written by the collection organizers at the ATCC and ECACC.

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Animal Cell Culture and Technology (THE BASICS (Garland Science)) by Michael Butler

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